Part:BBa_K2665015:Design

TDH3-AVP1-6xHis-CYC

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1341
  Illegal BglII site found at 2598
  Illegal BamHI site found at 2394
  Illegal BamHI site found at 3030
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 2690
  Illegal BsaI.rc site found at 2880
  

Design Notes

We added promoter and terminator in order to express the protein. We added We mistakenly added stop codon before His-tag.

Source

AVP1 is derived from Arabidopsis thaliana.